| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Harter, Abigail E (14)
|
PVNCAP |
54.72 |
54.71 |
-0.01 |
| 2 |
Pulley, Megan D (14)
|
VANOVA |
56.35 |
57.27 |
0.92 |
| 3 |
Standfield, Gabrielle R (14)
|
PVNCAP |
57.28 |
58.64 |
1.36 |
| 4 |
Belyakov, Catherine I (14)
|
PVRMSC |
58.72 |
58.65 |
-0.07 |
| 5 |
Kim, Olivia L (14)
|
MRLIAC |
59.98 |
59.90 |
-0.08 |
| 5 |
Fan, Julie (14)
|
PVRMSC |
59.30 |
59.90 |
0.60 |
| 7 |
Purnell, Catherine N (14)
|
PVNCAP |
58.58 |
1:00.01 |
1.43 |
| 8 |
Gregorace, Sophia R (14)
|
MRLIAC |
58.93 |
1:00.23 |
1.30 |
| 9 |
Ye, Camellia (14)
|
MRLIAC |
59.60 |
1:00.86 |
1.26 |
| 10 |
Rogers, Avery G (14)
|
VANOVA |
1:01.41 |
1:01.03 |
-0.38 |
| 11 |
Cosgrove, Kathleen G (14)
|
PVRMSC |
57.49 |
1:01.24 |
3.75 |
| 12 |
Kallman, Annalise F (14)
|
VANOVA |
1:01.08 |
1:03.91 |
2.83 |
| 13 |
Thomas, Avery L (14)
|
MADST |
1:07.84 |
1:06.81 |
-1.03 |
|
Competello, Madeline G (14)
|
MRLIAC |
1:00.81 |
1:04.01 |
3.20 |